Fluorescence microscopy today
- 1 December 2005
- journal article
- review article
- Published by Springer Nature in Nature Methods
- Vol. 2 (12) , 902-904
- https://doi.org/10.1038/nmeth1205-902
Abstract
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues. The impact of these and other new imaging methods in biophysics, neuroscience, and developmental and cell biology has been remarkable. Further advances in fluorophore design, molecular biological tools and nonlinear and hyper-resolution microscopies are poised to profoundly transform many fields of biological research.Keywords
This publication has 28 references indexed in Scilit:
- Optical sectioning microscopyNature Methods, 2005
- Fiber-optic fluorescence imagingNature Methods, 2005
- Deep tissue two-photon microscopyNature Methods, 2005
- A guide to choosing fluorescent proteinsNature Methods, 2005
- Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm LocalizationScience, 2003
- The neuronal naturalist: watching neurons in their native habitatNature Neuroscience, 2001
- THE GREEN FLUORESCENT PROTEINAnnual Review of Biochemistry, 1998
- Green Fluorescent Protein as a Marker for Gene ExpressionScience, 1994
- Two-Photon Laser Scanning Fluorescence MicroscopyScience, 1990
- Über Elementarakte mit zwei QuantensprüngenAnnalen der Physik, 1931