Reconstitution of catecholamine-sensitive adenylate cyclase activity: interactions of solubilized components with receptor-replete membranes.
- 1 September 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (9) , 3715-3719
- https://doi.org/10.1073/pnas.74.9.3715
Abstract
Membranes of mouse L cells that contain adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] but lack .beta.-adrenergic receptors were solubilized with Lubrol 12A9. Addition of such adenylate cyclase-containing extracts to .beta.-adrenergic receptor-replete membranes from adenylate cyclase-deficient S49 lymphoma cells resulted in the production of a catecholamine-sensitive adenylate cyclase system. The effects of .beta.-adrenergic agonists and antagonists on the reconstituted system reproduce those that are characteristic of the wild-type S49 lymphoma cell. The uncoupled variant of the S49 lymphoma contained adenylate cyclase, but donor extracts from this clone failed to reconstitute the hormone-sensitive enzyme activity when added to adenylate cyclase-deficient membranes. The uncoupled and adenylate cyclase-deficient variants of the S49 cell are not complementary.This publication has 20 references indexed in Scilit:
- Adenylate cyclase permanently uncoupled from hormone receptors in a novel variant of S49 mouse lymphoma cells.Proceedings of the National Academy of Sciences, 1977
- Expression of genes for metabolism of cyclic adenosine 3':5'-monophosphate in somatic cells. beta-Adrenergic and PGE1 receptors in parental and hybrid cells.Journal of Biological Chemistry, 1977
- beta-Adrenergic receptor: ligand binding properties and the interaction with adenylyl cyclase.1977
- Resolution of beta-adrenergic receptor binding and adenylate cyclase activity by gel exclusion chromatography.Journal of Biological Chemistry, 1977
- Coupling of catecholamine receptor from one cell with adenylate cyclase from another cell by cell fusion.Proceedings of the National Academy of Sciences, 1976
- Catecholamine-stimulated GTPase activity in turkey erythrocyte membranesBiochimica et Biophysica Acta (BBA) - Enzymology, 1976
- BETA-ADRENERGIC RECEPTORS AND ADENYLATE-CYCLASE - PRODUCTS OF SEPARATE GENES1976
- Thyroid-stimulating hormone binding to beef thyroid membranes. Relation to adenylate cyclase activity.1974
- Formation of a Novel Nucleotide by Fat Cell Preparations Containing Adenylate CyclaseJournal of Biological Chemistry, 1974
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951