Absence of a Low‐Molecular‐Weight DNA Polymerase from Nuclei of the Yeast, Saccharomyces cerevisiae
- 1 December 1974
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 50 (1) , 197-202
- https://doi.org/10.1111/j.1432-1033.1974.tb03888.x
Abstract
The DNA polymerase activity associated with purified nuclei of the yeast, Saccharomyces cerevisiae, was analyzed by sucrose gradient centrifugation. Using different salt concentrations there was no polymerase activity found sedimenting in the 3–4‐S region. On the contrary, the nuclear enzyme shows a main peak around 8 S, similar to the DNA polymerases from yeast cytoplasm. Other properties tested (e.g. behaviour on ion‐exchange columns, dependence of activity on salt concentration, inhibition by p‐chloromercuribenzoate etc.) were also found identical with those of the cytoplasmic DNA polymerases.This publication has 15 references indexed in Scilit:
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