Cloning and immunological characterisation ofEchinococcus granulosus ferritin
- 1 January 1995
- journal article
- Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 81 (5) , 382-387
- https://doi.org/10.1007/bf00931498
Abstract
A cDNA clone ofEchinococcus granulosus has been isolated from an expression library screened with sera from cystic echinococcosis patients. The deduced amino acid sequence shows 56% homology to the heavy chain of human ferritin.E. granulosus ferritin contains 173 amino acid residues and has a calculated molecular weight of 19830 Da and a statistical isoelectric point of 7.6. Functionally important amino acid residues of the ferroxidase centre are conserved in comparison with other ferritins. In vitro-translatedE. granulosus ferritin was tested for its diagnostic potential by immunoprecipitation. The antigenic reactivity exhibited a good potential for the further development ofE. granulosus ferritin as an immunodiagnostic tool for human hydatidosis.Keywords
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