Detection of BrdUrd incorporation in mammalian chromosomes by a BrdUrd antibody

1 July 1987

journal article
research article
Published by Springer Nature in Human Genetics

Vol. 76 (3) , 293-295
https://doi.org/10.1007/bf00283627

Abstract

A bromodeoxyuridine antibody staining technique (BAT) was applied for the analysis of human chromosomes of different chromosomal band resolution. For this purpose lymphocyte cultures were synchronized and labeled with bromodeoxyuridine during the second half of the S-phase. Generally BAT was found comparable to GTG banding though some prominent GTG bands and the constitutive heterochromatin exhibit less intense staining with this technique.

This publication has 12 references indexed in Scilit:

Influence of incorporated 5-bromodeoxyuridine on the frequencies of spontaneous and induced sister-chromatid exchanges, detected by immunological methods
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1986
Detection of bromodeoxyuridine incorporation in mammalian chromosomes by a bromodeoxyuridine antibody
Chromosoma, 1986
Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody
Human Genetics, 1986
MEASUREMENT OF SISTER CHROMATID EXCHANGES AT VERY LOW BROMODEOXYURIDINE SUBSTITUTION LEVELS USING A MONOCLONAL-ANTIBODY IN CHINESE-HAMSTER OVARY CELLS
1985
Considerations on the mechanism of differential Giemsa staining of BrdU-substituted chromosomes
Human Genetics, 1984
Decrease of DNA synthesis in amniotic fluid cells during the middle part of S-phase revealed by differential chromosome staining after incorporation of BrdU
Chromosoma, 1978
The use of antibody specific for bromodeoxyuridine for the immunofluorescent determination of DNA replication in single cells and chromosomes
Experimental Cell Research, 1975
Microfluorometric Detection of Deoxyribonucleic Acid Replication in Human Metaphase Chromosomes
Proceedings of the National Academy of Sciences, 1973
Asynchronous Duplication of Chromosomes in Cultured Cells of Chinese Hamster
The Journal of cell biology, 1960
THE ORGANIZATION AND DUPLICATION OF CHROMOSOMES AS REVEALED BY AUTORADIOGRAPHIC STUDIES USING TRITIUM-LABELED THYMIDINEE
Proceedings of the National Academy of Sciences, 1957