Identification of New Zealand wheat cultivars by reversed-phase high-performance liquid chromatography

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Abstract
Reversed-phase high-performance liquid chromatography (RP-HPLC) on columns containing large-pore pac kings gave excellent separation of alcohol-soluble wheat proteins (gliadins). Gliadin extracts, from the 10 most commonly grown New Zealand wheat cultivars of the 1985 – 86 season, were separated on a reversedphase (C8) column, eluted over 55 min with a solvent gradient of 28 - 50% acetonitrile ( + 0.1 % trifluoroacetic acid), and gliadins were detected at 210 nm. All 10 cultivars could be uniquely identified by this method. Growing environment and season had no major qualitative effect on the chromatographic pattern for any cultivar.