Enhancement of Histological Detail Using Metanil Yellow as Counterstain in Periodic Acid Schiff's Hematoxylin Staining of Glycol Methacrylate Tissue Sections

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Abstract
Histological detail in sections from tissues embedded in glycol methacrylate was improved by counterstaining PAS/iron-hematoxylin stained sections with a dilute solution of metanil yellow. The addition of the counterstain increases contrast in tissue sections and highlights PAS-positive entities. The staining protocol provides sharp definition of tissue morphology, differentiates cell types and other tissue components and does not produce background staining.