Uptake of 3H-GABA by the Purkinje cells of the rat embryo.

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Abstract
Uptake of 3H-GABA by the Purkinje cells in the rat embryo was examined by light microscopic and EM autoradiography. The developing Purkinje cells first became labeled with 3H-GABA at E[embryonic day]18 when they began to form the primordium of the Purkinje cell layer, and continued to be labeled until E-22 (at term). From E-18 to E-20 most of the Purkinje cells were heavily labeled, but at E-21 and E-22 the Purkinje cells situated at the top of the cerebellar gyri were heavily labeled while those situated at the bottom of the cerebellar sulci were not labeled. Silver grains on the labeled Purkinje cells were distributed on both the nucleus and the cytoplasm. At E-22 a few unlabeled axon terminals on the labeled Purkinje cell soma and also a few labeled glial processes in the Purkinje cell layer were first detected. Throughout the stages examined no silver grains were found on the external granular layer, on the molecular layer and on the intercellular spaces of the Purkinje cell layer and of the mantle layer. Developing Purkinje cells in rat embryo possess an ability of 3H-GABA uptake before synapses and/or glial wrapping around their soma are formed.