INHIBITION OF VIRUS MULTIPLICATION BY NAPHTHALENE ACETIC ACID IN TOBACCO TISSUE CULTURES AS REVEALED BY A SPECTROPHOTOMETRIC METHOD

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Abstract
A spectrophotometric method for the detn. of tobacco mosaic virus concn. in small tobacco stem tissue cultures is described. The preparatory step, involving incubation and dialysis in a salt-buffer mixture, is thought to utilize the principle of selective decomposition of the host nucleoproteins, leaving the virus nucleoprotein largely intact. The decomposition products of the host nucleoproteins are apparently removed during the dialysis. Folic acid, at the highest concn. that was nontoxic to the host, had no detectable effect on virus multiplication. Naphthalene acetic acid at a concn., of 10-4% decreased the virus concn. to approx. 1/3 that in control cultures.