Isolation of a cDNA clone for a catalytic subunit ofTorpedo marmorataacetylcholinesterase
- 2 December 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 193 (2) , 159-163
- https://doi.org/10.1016/0014-5793(85)80142-3
Abstract
We have constructed a cDNA library from Torpedo marmorata electric organ poly(A + ) RNA in the lambda phage expression vector λgtll. This library has been screened with polyclonal anti-acetylcholinesterase antibodies. One clone, λAChEl, produced a fusion protein which was recognized by the antibodies and which prevented the binding of native acetylcholinesterase in an enzymatic immune assay. These results indicate that λAChEl contains a cDNA insert coding for a part of a catalytic subunit of Torpedo acetylcholinesterase. The 200-base-pair cDNA insert hybridized to three mRNAs (14.5, 10.5 and 5.5 kb) from Torpedo electric organs. These mRNAs were also detected in Torpedo electric lobes.Keywords
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