Eclosion hormone of the silkworm Bombyx mori Expression in Escherichia coli and location of disulfide bonds
- 24 April 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 263 (2) , 358-360
- https://doi.org/10.1016/0014-5793(90)81413-i
Abstract
A gene encoding eclosion hormone (EH) from the silkworm, Bombyx mori was chemically synthesized, inserted into a secretion vector and expressed in Escherichia coli, leading to the production of biologically active EH. Sequence analysis of cystine-containing peptides in a thermolysin digest of this EH established the locations of 3 disulfide bonds in the molecule. Evidence was also obtained that the 6 residues at the NH2-terminal are dispensable but 4 residues at the COOH-terminal play an important role in EH activity.Keywords
This publication has 11 references indexed in Scilit:
- Isolation and primary structure of the eclosion hormone of the tobacco hornworm, Manduca sextaPublished by Elsevier ,2004
- Microanalysis of the amino acid sequence of the eclosion hormone from the tobacco hornworm Manduca sextaFEBS Letters, 1987
- Amino acid sequence of eclosion hormone of the silkworm, Bombyx mori.Agricultural and Biological Chemistry, 1987
- Synthesis and secretion of human epidermal growth factor by Escherichia coli.Proceedings of the National Academy of Sciences, 1985
- Secretion of Human Interferon-α Induced by Using Secretion Vectors Containing a Promoter and Signal Sequence of Alkaline Phosphatase Gene of Escherichia coliThe Journal of Biochemistry, 1985
- An evolutionary analysis of iso-is1 elements from Escherichia coli and Shigella strains.The Journal of General and Applied Microbiology, 1984
- Purification and properties of eclosion hormone of the silkworm, Bombyx mori.Agricultural and Biological Chemistry, 1983
- Nonenzymic reactivation of reduced bovine pancreatic ribonuclease by air oxidation and by glutathione oxidoreduction buffers.Journal of Biological Chemistry, 1975
- Isolation and structure of urogastrone and its relationship to epidermal growth factorNature, 1975
- The Release of Enzymes from Escherichia coli by Osmotic Shock and during the Formation of SpheroplastsJournal of Biological Chemistry, 1965