EVALUATION OF THE EFFECTS OF ADENOSINE ON HEPATIC AND ADIPOCYTE ADENYLATE-CYCLASE UNDER CONDITIONS WHERE ADENOSINE IS NOT GENERATED ENDOGENOUSLY
- 1 January 1979
- journal article
- research article
- Vol. 5 (4) , 289-302
Abstract
Direct effects of adenosine on adipocyte and hepatic adenylate cyclase were demonstrated in an assay system where adenosine is not generated. The substrate used, 2''-deoxy ATP may, on metabolism, only give rise to 2''-deoxyadenosine, which does not act at adenosine receptors. With a slight modification of existing assay techniques this assay system was used to detect a previously undiscovered adenosine receptor on rat liver plasma membranes, which is antagonized by methylxanthines and which stimulates adenylate cyclase activity in a GTP-dependent manner. The potent inhibitory effects of purine-modified adenosine analogs on fat cell adenylate cyclase are reproduced by adenosine in this assay system. An application of this approach to the study of adenylate cyclase simplifies detection of the role of adenosine and yields insights into the interaction between guanine nucleotides and hormones.This publication has 8 references indexed in Scilit:
- Adenosine analogs inhibit adipocyte adenylate cyclase by a GTP-dependent process: basis for actions of adenosine and methylxanthines on cyclic AMP production and lipolysis.Proceedings of the National Academy of Sciences, 1978
- Coupling of a single adenylate cyclase of two receptors: adenosine and catecholamineBiochemistry, 1978
- Two distinct adenosine-sensitive sites on adenylate cyclase.Proceedings of the National Academy of Sciences, 1977
- Activation of the hepatic adenylate cyclase system by divalent cations.Journal of Biological Chemistry, 1977
- ADENOSINE-SENSITIVE ADENYLATE-CYCLASE IN RAT STRIATAL HOMOGENATES AND ITS RELATIONSHIP TO DOPAMINE-SENSITIVE AND CA2+-SENSITIVE ADENYLATE CYCLASES1977
- Activation of steroidogenesis and adenylate cyclase by adenosine in adrenal and Leydig tumor cells.Journal of Biological Chemistry, 1976
- Adenosine-Mediated Stimulation of Bone Cell Adenylate Cyclase ActivityEndocrinology, 1976
- Regulation of adenylate cyclase from cultured human cell lines by adenosine.Journal of Biological Chemistry, 1976