Functional involvement of calcium in the homologous up‐regulation of the 1,25‐dihydroxyvitamin D3 receptor in osteoblast‐like cells
- 17 September 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 270 (1-2) , 165-167
- https://doi.org/10.1016/0014-5793(90)81258-p
Abstract
In several cell types 1,25‐dihydroxyvitamin D3 (1,25(OH)2D3) causes up‐regulation of its receptor. The present study demonstrates that in the osteoblast‐like cell line UMR 106 this up‐regulation is inhibited by two different calcium channel blockers (nitrendipine, verapamil). Also with chelating extracellular calcium (EGTA) and by inhibition of calcium release from intracellular stores (TMB‐8) comparable results were obtained. These findings indicate that calcium is functionally involved in this cellular response to the steroid hormone 1,25(OH)2D3. Moreover, data obtained with EGTA show that the 1,25(OH)2D3 receptor level is closely regulated by the extracellular calcium concentration.Keywords
This publication has 16 references indexed in Scilit:
- Role of calcium and cAMP in heterologous up-regulation of the 1,25-dihydroxyvitamin D3 receptor in an osteoblast cell lineCell Calcium, 1990
- Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in vitro.The Journal of cell biology, 1989
- Evidence that the self-induced metabolism of 1,25-dihydroxyvitamin D-3 limits the homologous up-regulation of its receptor in rat osteosarcoma cellsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1988
- 1,25-Dihydroxycholecalciferol-Dependent Calcium Uptake by Mouse Mammary Gland in Culture*Endocrinology, 1988
- Novel regulatory actions of 1α,25‐dihydroxyvitamin D3 on the metabolism of polyphosphoinositides in murine epidermal keratinocytesJournal of Cellular Physiology, 1987
- Rapid Changes in Skeletal Muscle Calcium Uptake Inducedin Vitroby 1,25-Dihydroxyvitamin D3Are Suppressed by Calcium Channel Blockers*Endocrinology, 1987
- Regulation of 1,25-Dihydroxyvitamin D3 Receptors by Vitamin D Analogs in Cultured Mammalian Cells*Endocrinology, 1985
- Genome-independent effects of 1,25-dihydroxy vitamin D-3 on membrane potentialBiochimica et Biophysica Acta (BBA) - Biomembranes, 1983
- Some statistical methods useful in circulation research.Circulation Research, 1980
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976