Biological activity of recombinant Ricinus communis agglutinin A chain produced in Escherichia coli
- 1 March 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 299 (3) , 209-212
- https://doi.org/10.1016/0014-5793(92)80116-x
Abstract
DNA encoding Ricinus communis agglutinin A chain was listed into the E. coli expression vector pDS S/3. Induced E. coli 71.18 cells which had been transformed with this plasmid express Ricinus communis agglutinin A chain in a soluble and biologically active form. Recombinant Ricinus communis agglutinin A chain had ribosomal RNA N‐glycosidase activity and was approximately 10‐fold less active than ricin A chain in a cell‐free protein synthesis inhibition assay.Keywords
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