Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination.
Open Access
- 1 March 1997
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 7 (3) , 268-280
- https://doi.org/10.1101/gr.7.3.268
Abstract
The detailed genomic organization of a gene-dense region at human chromosome 12p13, spanning 223 kb of contiguous sequence, was determined. This region is composed of 20 genes and several other expressed sequences. Experimental tools including RT-PCR and cDNA sequencing, combined with gene prediction programs, were utilized in the analysis of the sequence. Various computer software programs were employed for sequence similarity searches and functional predictions. The high number of genes with diverse functions and complex transcriptional patterns make this region ideal for addressing challenges of gene discovery and genomic characterization amenable to large-scale sequence analysis.Keywords
This publication has 47 references indexed in Scilit:
- tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic SequenceNucleic Acids Research, 1997
- Pressing ahead with human genome sequencingNature Genetics, 1995
- Identification of Protein Coding Regions In Genomic DNAJournal of Molecular Biology, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- The Primary Structure of Rat Ribosomal Protein L13Biochemical and Biophysical Research Communications, 1994
- Striking sequence similarity over almost 100 kilobases of human and mouse T–cell receptor DNANature Genetics, 1994
- Prediction of gene structureJournal of Molecular Biology, 1992
- Isolation and characterization of a novel gene with differential expression in benign and malignant human breast tumoursHuman Molecular Genetics, 1992
- Basic Local Alignment Search ToolJournal of Molecular Biology, 1990
- Identification of the Human U7 snRNP as One of Several Factors Involved in the 3′ End Maturation of Histone Premessenger RNA'sScience, 1987