EFFECT OF THE ANTITUMOR ANTIBIOTIC NEOCARZINOSTATIN ON DNA SYNTHESIS IN VITRO

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Abstract
The effect of neocarzinostatin on in vitro DNA synthesis by DNA polymerase I prepared from Escherichia coli was investigated. DNA synthesis in vitro by Escherichia coli polymerase I was inhibited in the presence of neocarzinostatin. The inhibition increased when the template was preincubated with the antibiotic. Synthesis directed by poly d(A-T) was more sensitive to the antibiotic than synthesis directed by calf thymus DNA. The melting temperature of DNA decreased whenit was incubated with neocarzinostatin in vitro. From these results it was suggested that the effect of neocarzinostatin on DNAsynthesis in vitro could be caused by a direct interaction of the antibiotic with the template.