Cloning and expression of an α-amylase encoding gene from the hyperthermophilic archaebacterium Thermococcus hydrothermalis and biochemical characterisation of the recombinant enzyme
- 1 May 2000
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 186 (1) , 67-71
- https://doi.org/10.1016/s0378-1097(00)00117-8
Abstract
An α-amylase encoding gene from the extremely thermophilic Archaea Thermococcus hydrothermalis was cloned and expressed in Escherichia coli . The encoded α-amylase possesses molecular characteristics specific to the Archaea, especially from Pyrococcus species, with biochemical characteristics of the α-amylases from Thermococcus . The gene is 1374 bp long and encodes a protein of 457 amino acids composed of a 22 amino acid putative signal peptide and a 435 amino acid mature protein (calculated molecular mass 49 236 Da). The T. hydrothermalis recombinant α-amylase is optimally active at 75–85°C and at pH 5.0–5.5.Keywords
This publication has 0 references indexed in Scilit: