Abstract
Two ovariectomized cows were injected intramuscularly once daily with 300 i.u. of ACTH*) on three consecutive days. The urine was collected with the aid of rubber mats. The method for oestrogen analysis comprised acid hydrolysis of the urine, saponification of the phenolic fraction, inethylation and column chromatography followed by fluorimetry (Lunaas 1964 and unpublished).

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