Activated Ras interacts with the Ral guanine nucleotide dissociation stimulator.
Open Access
- 8 November 1994
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (23) , 11089-11093
- https://doi.org/10.1073/pnas.91.23.11089
Abstract
The yeast two-hybrid system was used to identify proteins that interact with Ras. The H-Ras protein was found to interact with a guanine nucleotide dissociation stimulator (GDS) that has been previously shown to regulate guanine nucleotide exchange on another member of the Ras protein family, Ral. The interaction is mediated by the C-terminal, noncatalytic segment of the RalGDS and can be detected both in vivo, using the two-hybrid system, and in vitro, with purified recombinant proteins. The interaction of the RalGDS C-terminal segment with Ras is specific, dependent on activation of Ras by GTP, and blocked by a mutation that affects Ras effector function. These characteristics are similar to those previously demonstrated for the interaction between Ras and its putative effector, Raf, suggesting that the RalGDS may also be a Ras effector. Consistent with this idea, the RalGDS was found to inhibit the binding of Raf to Ras.Keywords
This publication has 25 references indexed in Scilit:
- Gap Domains Responsible for Ras P21-Dependent Inhibition of Muscarinic Atrial K + Channel CurrentsScience, 1992
- The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.Proceedings of the National Academy of Sciences, 1991
- The C‐terminal part of the CDC25 gene product plays a key role in signal transduction in the glucose‐induced modulation of cAMP level in Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1990
- Mutations of the Adenylyl Cyclase Gene that Block RAS Function in Saccharomyces cerevisiaeScience, 1990
- High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrierCurrent Genetics, 1989
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1989
- ras GENESAnnual Review of Biochemistry, 1987
- In yeast, RAS proteins are controlling elements of adenylate cyclasePublished by Elsevier ,1985
- Functional homology of mammalian and yeast RAS genesCell, 1985