Regeneration of Whole Plants from Isolated Stem Parenchyma Cells of Solanum sisymbriifolium1

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Abstract
Embryogenic callus was induced when pith of Solanum sisymbriifolium Lam. was cultured on Linsmeier-Skoog (LS) medium supplemented with (6-[(3,3-dimethylallyl) amino]-purine) (2iP), indole-3-acetic acid (IAA), and (2,4-dichlorophenoxy) acetic acid (2,4-D). Combinations of 2iP and IAA were tested for best growth rate of stock callus cultures. Differentiation of leaflets was induced when explants were grown on LS medium with high concentrations of 2iP and low IAA. Roots were formed upon transfer to Nitsch's medium that contained IAA. Morphologic variations in the leaves and cytological studies of callus-induced plants indicated that they were genetically aberrant. Callus-induced plants retained their resistance to the root-knot nematode, Meloidogyne incognita.

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