Dietary Lutein But Not Astaxanthin or β-Carotene Increases pim-1 Gene Expression in Murine Lymphocytes
- 1 March 1999
- journal article
- Published by Taylor & Francis in Nutrition and Cancer
- Vol. 33 (2) , 206-212
- https://doi.org/10.1207/s15327914nc330214
Abstract
This study investigates the effect of dietary carotenoids on pim-1 gene expression in mouse splenocytes. Female BALB/c mice were fed 0%, 0.02%, or 0.4% astaxanthin, β-carotene, and lutein for two weeks. Plasma and liver were obtained for the analysis of carotenoids. Splenocytes were isolated and cultured in the presence of concanavalin A, and the level of pim-1 mRNA was determined by Northern blot analysis. None of the carotenoids were detectable in the plasma and liver of unsupplemented mice. In plasma the concentration of astaxanthin (4.9-54.7 μmol/l) was dramatically higher than that of lutein (1.4-2.0 μmol/l) and β-carotene (0.1-0.7 μmol/l). Carotenoid uptake by the spleen but not the liver reflected that observed in plasma. In mice fed 0.4% of each carotenoid, the absolute concentration of the carotenoid in the liver was highest for astaxanthin (24 nmol/g) followed by b-carotene (7.5 nmol/g) and lutein (1.58 nmol/g). Mice fed lutein showed a dose-related increase in pim-1 mRNA expression. The steady-...Keywords
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