THE EFFECT OF INFECTION BY HELMINTHOSPORIUM SATIVUM ON THE AMINO ACID CONTENT OF WHEAT ROOTS

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Abstract
The amino acids present in roots of healthy and diseased (Helminthosporium sativum P. K. & B.) wheat seedlings were determined by paper chromatography. Treatment of dried roots with 80% ethanol followed by acid hydrolysis effected maximum extraction of the amino acids. Eleven free and 13 combined amino acids were identified on the chromatograms, and four unidentified fractions were indicated. Total nitrogen of the diseased roots was higher on a dry weight basis than that of healthy roots. The aggregate content of both free and combined amino acids was slightly higher in the diseased roots than in the healthy ones. However, the concentrations of free alanine, serine, and asparagine in the diseased roots were twice those in the healthy ones.