Mucosal glycoprotein was isolated from human intestinal mucosa. It possessed higher sialic acid and sulfate contents than those of the gastric mucosal glycoprotein. The former was, therefore, stainable with Alcian Blue while the latter was not. These two kinds of glycoproteins gave a specific precipitate line against the corresponding antiserum, respectively, on the Ouchterlony assay. Using the direct immunofluorescent antibody technique, the intestinal glycoprotein was demonstrated to distribute in the alcianophilic goblet cells throughout the intestinal mucosa but only faintly demonstrable in the surface epithelium of gastric mucosa.