Separation of Human Cells Bearing HLA—DR Antigens Using a Monoclonal Antibody Resetting Method

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Abstract
A technique is described for enriching, from human blood, cells bearing HLA—DR antigens. The method depends on the use of monoclonal mouse antibody which reacts with HLA—DR structures. Cells to which this antibody has bound can be separated after resetting with bovine erythro-cytes coated with anti-mouse immunoglobulin. The cells thus enriched may be used for HLA—DR typing by standard cytotoxicity methods with allogeneic sera.