Cytochemical Localization of Plasma Membrane Enzyme Markers During Interiorization of Tachyzoites of Toxoplasma gondii by Macrophages

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Abstract
The enzyme activity of Mg‐ATPase, Na+‐K+‐ATPase, 5′‐nucleotidase and NAD(P)H‐oxidase was cytochemically detected at the ultrastructural level in mouse peritoneal macrophages infected with untreated and with specific antibody‐coated Toxoplasma gondii tachyzoites. The Mg++‐ATPase and 5′‐nucleotidase were distributed throughout the macrophages’plasma membrane but were not observed in the membrane lining endocytic vacuoles containing ingested parasites; however, Na+‐K+‐ATPase activity was detected in the macrophages’plasma membrane as well as in the parasitophorous vacuoles that contained untreated or specific antibody‐coated parasites. Reaction product, indicative of NAD(P)H‐oxidase, was detected in the parasitophorous vacuoles that contained only‐specific antibody‐coated parasites.

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