Follicular Reorganization and131I Utilization by Cultured Rat Thyroid Cells Implanted Into Thyroidectomized Rats

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Abstract
Rat thy-roid gland cells were isolated by a trypsinization procedure and cultured for 3 days either in Gelfoam pads in a medium with no TSH [thyro-tropin] or as monolayers in a medium containing 0.17 U of bovine TSH per ml. The cells cultured by the 1st method were implanted into rats 52-217 days after they had been thyroidectomized by surgical removal of the gland at 20[long dash]25 days of age followed by an 131I injection. The cells cultured by the 2nd method were implanted into rats 19 and 20 days after they had been thyroidectomized by an 131I injection on the day of birth. In both cases the implanted cells reorganized into aggregations of follicles containing colloid in their lumens. Autoradto-graphs of sections of these reformed follicles revealed the presence of protein-bound 131I in the colloid. The implants in both groups of animals utilized radio iodine and formed labeled MIT [monolodotyrosine], DIT [diiodotyrosine], T4 [thyroxine], and T3 [triiodothyronine]. Iodine metabolism of the implants in the 2nd group of animals was similar to that observed in intact thyroid glands of normal rats of the same strain, including the release of labeled thyroxine into the circulation.

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