Transformation of colonial Escherichia coli on solid media
Open Access
- 1 July 2004
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 236 (1) , 61-64
- https://doi.org/10.1016/j.femsle.2004.05.023
Abstract
We report that colonial Escherichia coli cells on various solid media can develop modest genetic competence. Using an on-filter culture system, we found that E. coli colonies on CaCl2-containing agar were transformed in the presence of plasmid DNA. Interestingly, transformation also occurred on LB-agar, various moist foods and even on H2O-agar. These results suggest that some populations of colonial E. coli in various environments could become transformable regardless of the surrounding Ca2+ concentration.Keywords
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