Mitochondrial swelling associated with exercise and method of fixation

Publisher Website
Google Scholar
Abstract
This study was undertaken to determine the effects of exercise and-fixation procedures on the morphology of skeletal muscle mitochondria. Both trained and untrained rats were run on a motor driven treadmill. The velocities of the runs were varied to produce exhaustion over a wide range of durations from 9 to 125 minutes. Buns were also terminated before exhaustion occurred, Portions of the soleus and gastrocnemius muscles were excised and prepared for electron microscopial examination. No mitochondrial swelling was observed in any of the tissues when a cacodylate buffered glutaraldehyde and post osmium fixation was used. However, when adjacent sections of muscle from exhausted rats were fixed in veronal buffered osmium, gross ultrastructural changes did occur. This fixation procedure was not associated with ultrastructural change in muscle from resting animals, however, suggesting that mitochondria from exhausted animals are more susceptible to fixation artifact than are the mitochondria from resting animals.

This publication has 0 references indexed in Scilit: