Detection of Bacteria in Environmental Samples by Direct PCR Without DNA Extraction
Open Access
- 5 September 2001
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 31 (3) , 598-607
- https://doi.org/10.2144/01313rr04
Abstract
Cultured cells and environmental samples were used directly in PCRs without the isolation of DNA. Serial dilution was used to eliminate the inhibitory effect of materials in natural samples. Primer...Keywords
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