Nuclear protein factors binding to a class I patatin promoter region are tuber-specific and sucrose-inducible

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Abstract
Genes encoding patatin, the major storage protein of the potato tuber, are generally divided into two classes, class I and class II. The expression of the class I patatin genes is normally tuber-specific, but can be induced in leaves by high concentrations of sucrose. By employing electrophoretic mobility shift assays (EMSA), we have identified nuclear protein factors that interact specifically with the proximal portion of the class I patatin promoter that is required for tuber-specific and sucrose-inducible expression. The factors were detected in nuclear extracts prepared from potato tubers and sucrose-induced leaves, but not in extracts from leaves of normal potato plants. Four putative transcription factor-binding sites were localized using DNase I footprinting. Competitive EMSA was employed to show that the same protein factor binds to at least two of the sites (boxes D and M). Interestingly, these two binding sites are highly homologous to light-responsive elements present in genes for theribulose-1,5-bisphosphate carboxylase small subunit.