Effect of unlabeled helper probes on detection of an RNA target by bead-based sandwich hybridization
Open Access
- 1 January 2004
- journal article
- research article
- Published by Taylor & Francis in BioTechniques
- Vol. 36 (1) , 124-132
- https://doi.org/10.2144/04361rr03
Abstract
Unlabeled helper oligonucleotides assisting a bead-based sandwich hybridization assay were tested for the optimal placement of the capture and detection probes. The target used was a full-length in... Helper Probes Chip In Annealing of a “helper” oligonucleotide probe directly adjacent to a labeled detection probe can enhance hybridization specificity and signal intensity in various nucleic acid...Keywords
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