MICROBIOLOGICAL ASPECTS OF THE DIGLUTAMYL DERIVATIVES OF CITROVORUM FACTOR AND N 10 FORMYLFOLIC ACID

Abstract
Preparations of diglutamyl derivatives of citrovorum factor and N10-formyl-folic acid obtained from extracts of Clostridium cylindiosporum do not possess significant growth activity for Streptococcus faecalis. The citrovorum factor polyglutamate has partial activity for Leuconostoc citrovorum. Both triglutamates exhibit essentially full activity for Lactobacillus casei. After digestion of both compounds with rat liver, all of the theoretical activity becomes available to S. faecalis and L. citrovorum. Digestion with chicken pancreas conjugase results in significant, but incomplete, release of activity for these 2 organisms.