A Jun-binding protein related to a putative tumor suppressor.
- 15 July 1993
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (14) , 6726-6730
- https://doi.org/10.1073/pnas.90.14.6726
Abstract
A lambda gt11 cDNA library of chicken embryo fibroblasts was screened with biotinylated Jun protein to identify Jun-binding clones. Eight such clones were isolated; one contains a gene referred to as jif-1 that is homologous to the putative tumor suppressor gene QM. jif-1 codes for a protein of 25 kDa that binds to the leucine zipper of viral and cellular Jun. The Jif-1 protein also binds to itself. Jif-1 does not contain a leucine zipper, and it does not bind to the 12-O-tetradecanoylphorbol 13-acetate response element DNA sequence. Complex formation of Jif-1 with Jun inhibits DNA binding and reduces transactivation by Jun. Addition of Fos protein to Jun-Jif-1 complexes restores DNA-binding activity. These observations suggest that Jif-1 is a negative regulator of Jun.Keywords
This publication has 41 references indexed in Scilit:
- The cell-type-specific activator region of c-Jun juxtaposes constitutive and negatively regulated domains.Genes & Development, 1992
- Identification and characterization of a new gene in the human Xq28 regionHuman Molecular Genetics, 1992
- cDNA cloning and genomic analysis of a new multigene family sharing common phylogenetic and expression profiles with the laminin receptor geneBiochemical and Biophysical Research Communications, 1992
- Cross-coupling of signal transduction pathways: zinc finger meets leucine zipperTrends in Genetics, 1991
- Phosphorylation of c-jun mediated by MAP kinasesNature, 1991
- Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domainNature, 1991
- IP-1: A dominant inhibitor of Fos/Jun whose activity is modulated by phosphorylationCell, 1991
- Control of c-Jun activity by interaction of a cell-specific inhibitor with regulatory domain δ: Differences between v- and c-JunCell, 1990
- Redox Regulation of Fos and Jun DNA-Binding Activity in VitroScience, 1990
- Biochemical analysis of transcriptional activation by Jun: Differential activity of c- and v-JunCell, 1989