Effect of 2-Mercaptoethanol on Rabbit Pancreas Isoantibodies.

Publisher Website
Google Scholar
Abstract
Sucrose density gradient ultracentrifugation has shown that anti-rabbit pancreas rabbit isoantibodies could be classed with the 7S globulins. In agar electrophoresis, the anti-rabbit pancreas rabbit antibody had a mobility of 1 globulin. These antibodies were sensitive to reduction by 0.1 [image] mercaptoethanol. Reduction with mercaptoethanol and alkylation with iodo-acetamide did not alter their sedimentation coefficient or precipitability by goat antiserum. There was, however, a total loss of complement fixation ability, and marked reduction in both passive cutaneous anaphylactic and precipitating abilities. The degree of reduction of these 2 abilities seemed to depend upon the height of the titer before treatment. The greater the titer, the more residual antibody after reduction. Naturally occurring antibodies to gastrointestinal mucosa also sedimentated as 7S globulin and were inactivated by mercaptoethanol. 7S antibody to bovine serum albumin was mercaptoethanol resistant.

This publication has 0 references indexed in Scilit: