Effect of low temperature and sucrose on bulb development and on the carbohydrate status of bulbing shoots of tulipin vitro

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Abstract
Shoots produced from tulip floral stem explants in vitro and incubated at 20°C for 12 to 16 weeks are capable of initiating a bulb primordium at their base. Development of this pri- mordium was enhanced when cultures were subcultured onto medium containing 6% sucrose before incubation at 4°C for 12 weeks followed by final incubation at 20 or 25°C for 20 weeks. Sucrose appeared to be an important type of carbohydrate involved in bulb development. Low-temperature incubation of shoot cultures enhanced bulb development and was associated with a high level of sucrose in the shoots before they were transferred to the final incubation at 20 or 25°C.