Detection of varicella-zoster virus (VZV) DNA in clinical samples from patients with VZV by the polymerase chain reaction
- 1 January 1991
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 29 (1) , 76-79
- https://doi.org/10.1128/jcm.29.1.76-79.1991
Abstract
A polymerase chain reaction system for the detection of varicella-zoster virus was established. Of 25 nucleotides, 4 oligonucleotide pairs (regions of thymidine kinase, thymidylate synthetase, glycoprotein I, and immediate early gene) were synthesized. The first three oligonucleotide pairs could be used as primers on the basis of specific DNA amplification. Varicella-zoster virus DNA was amplified by this polymerase chain reaction system in 20 of 20 vesicle samples, 5 of 6 crusts, and 12 of 13 throat swabs collected from patients with clinical varicella. ImagesKeywords
This publication has 9 references indexed in Scilit:
- Study of Virus Isolation From Pharyngeal Swabs in Children With VaricellaArchives of Pediatrics & Adolescent Medicine, 1989
- Transcription Mapping of Glycoprotein I (gpI) and gpIV of Varicella‐Zoster Virus and Immunological Analysis of the gpI Produced in Cells Infected with the Recombinant Vaccinia VirusMicrobiology and Immunology, 1989
- Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor δ ChainScience, 1989
- Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.Proceedings of the National Academy of Sciences, 1988
- Thermostable DNA polymerase chain amplification of t(14;18) chromosome breakpoints and detection of minimal residual disease.Proceedings of the National Academy of Sciences, 1988
- The Complete DNA Sequence of Varicella-Zoster VirusJournal of General Virology, 1986
- Viremia is present in incubation period in nonimmunocompromised children with varicellaThe Journal of Pediatrics, 1985
- Viremic phase in nonimmunocompromised children with varicellaThe Journal of Pediatrics, 1984
- Isolation of varicella-zoster virus from pharyngeal and nasal swabs in varicella patients.1984