Inhibitory effect of cadmium on vitamin D-stimulated calcium transport in rat duodenum in vitro.

Abstract
The in vitro effect of Cd [environmental pollutant] on vitamin D-stimulated Ca transport in the rat was studied using the everted gut sac technique. Ca transport was significantly inhibited by the addition of Cd to the mucosal incubation medium. The kinetic analysis of the inhibitory effect of Cd on Ca transport revealed that the type of inhibition was competitive. The apparent Km and Vmax values for Ca were 1.0 mM, and 2.86 .mu.mol ml-1 .cntdot. hr-1, respectively. An apparent Ki for Cd was 0.02 mM.

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