DNA Polymerase Activities in Virions of Visna Virus, a Causative Agent of a “Slow” Neurological Disease
- 1 January 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (1) , 182-186
- https://doi.org/10.1073/pnas.68.1.182
Abstract
The presence is reported of an RNA-instructed DNA polymerase in visna virus, the causative agent of a “slow” neurological disease in sheep. The product synthesized by the RNA-directed reaction has been shown to be a DNA heteropolymer by the following criteria: synthesis requires the presence of all four deoxyriboside triphosphates; the product is resistant to ribonuclease and alkali but is degraded by DNase; and the product has a density of 1.420 in Cs 2 SO 4 solution, characteristic of DNA. Visna virions, like those of the oncogenic RNA viruses, contain DNA polymerase activities that respond to a variety of double-stranded DNAs and to synthetic DNA·RNA hybrids.Keywords
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