Antibody Response, Delayed Hypersensitivity, and Immunity in Guinea Pigs Induced by Smooth and Rough Strains of Brucella abortus
- 1 July 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 124 (1) , 47-57
- https://doi.org/10.1093/infdis/124.1.47
Abstract
The abilities of attenuated smooth (strain 19) and rough (strain 45/20) Brucella abortus to immunize guinea pigs were tested 20 weeks after vaccination by respiratory challenge with virulent (strain 2308) B. abortus. The two vaccine strains were presented as living organisms in a small dose (106 organisms) or as killed organisms in a large dose (1010 organisms) in Freund's incomplete adjuvant. All vaccines gave significant protection; the superiority of living strain 19 was demonstrable only at a low level of challenge. In parallel studies with different animals, vaccinated guinea pigs were tested for delayed hypersensitivity. Animals given all the vaccines had comparable skin reactions after intradermal injection of brucellergen. All of the vaccines primed animals for the production of IgG against smooth antigen following skin-test with brucellergen. Long-lasting immunity to infection was conferred only by vaccines that induced delayed hypersensitivity and primed for antibody production, but it was not possible to dissociate these effects.Keywords
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