Citrus isozymes

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Abstract
Citrus leaf extracts were analyzed by starch gel electrophoresis for isozymes of hexokinase (HK), isocitrate dehydrogenase (IDH), leuclne aminopeptldase (LAP), malate dehydrogenase (MDH), and malic enzyme (ME). The first three showed a single region of activity suggesting each is specified by a single gene. Banding patterns indicated HK and LAP are monomeric, and IDH is dimeric. Evidence of four alleles of Hk and ldh and three of Lap was found in the nine species of cultivated Citrus and Poncirus trifoliata. Isozymes of MDH occurred in three gel regions, are apparently specified by four genes two of which, Mdh-1 and Mdh-2, are polymorphic. Zymograms indicated that MDH is dimeric but the subunits of Mdh-1 do not dimerize with those of Mdh-2. ME is specified by two genes but only one was polymorphic. ME is apparently monomeric. Isozymes of citrus provide molecular tags to determine the genetic origin of citrus seedlings that can be either asexual from the nucellus or sexual from a zygote. A very large proportion of all possible seedlings either from selfing or from crossing can now be distinguished as to their genetic origin with isozyrnes.

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