Experimental infection and the state of viral latency of adult Tupaia with herpes simplex virus type 1 and 2 and infection of Juvenile Tupaia with temperature-sensitive mutants of HSV type 2

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Abstract
Summary The susceptibility of adultTupaia belangeri to infection with herpes simplex virus (HSV) was investigated. Adult animals were inoculated intraperitoneally with HSV type 1 or 2. With the exception of HSV-2, strain HG-52, 105–106 PFU of all HSV strains caused lethal infection irrespective of the age of the animals. Infectious HSV was recovered from the spinal cord of those animals which had survived infection with a low dose of virus. The DNA of the recovered viruses was compared to the DNA of the inoculated HSV. The viral genome of the recovered HSV was unchanged as judged by analysis of the fragment pattern of the viral DNAs using restriction endonucleases. Animals which had survived the first HSV infection were protected against a second infection even at highly lethal doses of HSV-1 or 2. Juvenile Tupaia survived infection with temperature-sensitive mutants of HSV-2, strain HG-52, which induced protection against a second infection with lethal doses of HSV-1 or 2.