N.m.r. and e.p.r. characterization of [4-carboxy-2,6-dinitrophenyl-lysine]cytochromes c
- 1 March 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 193 (3) , 1021-1024
- https://doi.org/10.1042/bj1931021
Abstract
Monosubstituted [4-carboxy-2,6-dinitrophenyl-lysine]cytochromes c were investigated by n.m.r. and e.p.r. Modification of Lys-13 or Lys-72 in ferricytochrome c by 4-chloro-3,5-dinitrobenzoate yields either of two different conformers that are rapidly exchanging in the native form. The equilibrium involves small local changes in the conformation of Met-80 (the sixth ligand) and Phe-82, as a result of whether Lys-13 is the ‘on’ or ‘off’ position in the Lys-13–Glu-90 salt bridge.This publication has 16 references indexed in Scilit:
- Perchlorate Binding to Cytochrome cEuropean Journal of Biochemistry, 1980
- Mapping of the interaction domain for purified cytochrome c1 on cytochrome cFEBS Letters, 1980
- Electrostatic interactions in cytochrome c. The role of interactions between residues 13 and 90 and residues 79 and 47 in stabilizing the heme crevice structure.Journal of Biological Chemistry, 1980
- Different chirality of the axial methionine in homologous cytochromes c determined by 1H NMR and CD spectroscopyBiochemical and Biophysical Research Communications, 1980
- Evolutionary change of the heme C electronic structure: Ferricytochrome c-551 from Pseudomonas aeruginosa and horse heart ferricytochrome cBiochemical and Biophysical Research Communications, 1978
- [18] Mitochondrial cytochrome c: Preparation and activity of native and chemically modified cytochromes cPublished by Elsevier ,1978
- Multiple low spin forms of the cytochrome c ferrihemochrome. EPR spectra of various eukaryotic and prokaryotic cytochromes c.Journal of Biological Chemistry, 1977
- Tuna cytochrome c at 2.0 A resolution. II. Ferrocytochrome structure analysis.Journal of Biological Chemistry, 1977
- Pulsed NMR Study of the Structure of Cytochrome cCold Spring Harbor Symposia on Quantitative Biology, 1972
- Some aspects of pH and temperature dependence of the NMR spectra of cytochrome cBiochemical and Biophysical Research Communications, 1971