31P NMR measurements of intracellular pH and 2,3-diphosphoglycerate in erythrocytes
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 47 (3) , 233-237
- https://doi.org/10.3109/00365518709168896
Abstract
31P nuclear magnetic resonance (NMR) spectroscopic determinations of 2,3-diphosphoglycerate (2,3-DPG) concentrations in erythrocytes have been compared with values obtained by an enzymatic assay. It was found that the mean difference over a concentration range from 2 to 20 mmol 2,3-DPG/1 red blood cells (RBC) was 0.18±0.39 (SEM) mmol/l RBC (n=10). 31P NMR spectroscopy was further tested by simultaneous measurements of 2,3-DPG and intracellular pH under conditions where the concentration of 2,3-DPG was varied. Thereby a linear relation between the pH gradient (ΔpH) over the erythrocyte membrane and 2,3-DPG concentrations was found. The data fit a Donnan equilibrium model for the pH difference over the erythrocyte membrane. We conclude that 31P NMR spectroscopy is a sensitive and accurate method that makes possible simultaneous and non-invasive measurements of intracellular pH and 2,3-DPG.Keywords
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