Incorporation Rates of [1‐14C]Glycerol into the Molecular Species of Alkyl Ether Phospholipids of Ehrlich Ascites Tumor Cells in vivo

Open Access
Publisher Website
Google Scholar
Abstract
[1-14C]Glycerol was injected into the peritoneal cavity of mice bearing Ehrlich ascites tumor cells and the lipids were extracted from the cells after selected periods. 1-0-Alkyl-2-acyl-3-acetylglycerols and 1,2-diacyl-3-acetylglycerols were prepared from the ethanolamine and choline phosphoglycerides and fractionated by AgNO3-impregnated TLC into 7 molecular species. Bands 1-7 obtained in this way can be designated as saturated, monoene, diene (11), diene (02), triene, tetrene and pentaene + hexaene species, respecitvely. Band 7, composed of hexaene species, was predominent among those derived from ether phospholipids. The predominant species was band 4 (satured/linoleate) in diacyl ethanolamine and choline phospholipids. The specific radioactivities of the fractionated species were determined. In diacyl or alkyl ether compounds of ethanolamine and choline phosphoglycerides, disaturated species turned over rapidly, while in alkyl ether ethanolamine phosphoglyceride, hexaene + pentaene molecular species turned over rapidly. In diacyl compounds of the ethanolamine phosphoglyceride, hexaene molecular species also turned over at a high rate. In diacyl ethanolamine or choline phosphoglycerides, dienoic species turned over slowly.

This publication has 16 references indexed in Scilit: