Improved radioimmunoassay for 11-deoxycortisol (compound S) in plasma.
- 1 October 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (10) , 1704-1707
- https://doi.org/10.1093/clinchem/25.10.1704
Abstract
We describe a radioimmunoassay for 11-deoxycortisol (Compound S) that involves preliminary chromatography on Sephadex LH-20, to isolate 11-deoxycortisol from crossreacting structurally related steroids. Pools with means of 3.4, 12.9, and 69.9 micrograms/L gave intra-assay coefficients of variation of 9.7, 9.6, and 11.4%, respectively; pools with means of 4.6, 27, and 95 micrograms/L gave inter-assay CV's of 10.1, 9.7, and 6.2%, respectively. A normal range (mean +/- 2SD) of 0.5 to 12.0 micrograms/L was obtained. This procedure is useful for the specific determination of 11-deoxycortisol, commonly determined in blood as part of the metyrapone test for assessing pituitary corticotropin reserve.This publication has 6 references indexed in Scilit:
- A simple radioimmunoassay for plasma cortisol and 11-deoxycortisol (17,21-dihydroxy-4-pregnene-3,20-dione)Steroids, 1976
- MEASUREMENT OF CORTISOL, CORTISONE, 11-DEOXYCORTISOL AND CORTICOSTERONE IN FOETAL SHEEP PLASMA DURING THE PERINATAL PERIODJournal of Endocrinology, 1976
- SERUM CORTISOL AND 11-DESOXYCORTISOL LEVELS IN HIRSUTE PREMENOPAUSAL WOMEN1976
- Simplified Assessment of Pituitary-Adrenal ReserveAnnals of Internal Medicine, 1971
- CLINICAL APPLICATION OF A NEW TEST OF PITUITARY RESERVE*Journal of Clinical Endocrinology & Metabolism, 1959
- The Use of Probability Paper for the Graphical Analysis of Polymodal Frequency DistributionsJournal of the Marine Biological Association of the United Kingdom, 1949