Abstract
Neurofibrillar bundles were isolated from the nervous system of the turtle leech (Macrobdella sp.) and stained negatively with 1% Na-phosphotungstate solution at pH 7.3. Electron microscopy of the preparations reveals a hierarchically ordered substructure. Heavily beaded filaments of about 40 A in diameter are associated in filamentous aggregates measuring mostly about 300 A in diameter. The filamentous aggregates are intertwined into larger superhelices and associated in different degrees into neurofibrillar bundles.