Some features of the bacterial membrane studied with the aid of a new fractionation technique
- 1 April 1971
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 122 (2) , 197-207
- https://doi.org/10.1042/bj1220197
Abstract
Membranes of Bacillus megaterium and other bacteria were bound to crystals of cadmium lauroylsarcosinate and were resolved into nine lipoprotein fractions by elution with potassium chloride and sodium deoxycholate solutions. The fractions differed widely in protein/lipid ratio. Some major protein species were probably common to all the fractions, but the phospholipid composition showed some variation. Electron-microscopic examination after negative staining revealed that material in certain fractions was in the form of particles of diameter about 12nm, and other fractions consisted of amorphous aggregates. The composition of the membrane in terms of the nine fractions was influenced markedly by the conditions of culture of the bacteria. Radioactive-labelling experiments suggested that some fractions served as precursors of others, and one fraction appeared to contain the attachment points of the DNA.Keywords
This publication has 16 references indexed in Scilit:
- Binding of Dodecyl Sulfate to Proteins at High Binding Ratios. Possible Implications for the State of Proteins in Biological MembranesProceedings of the National Academy of Sciences, 1970
- Lipid synthesis in relation to the cell cycle of Bacillus megaterium KM and Escherichia coliBiochemical Journal, 1969
- Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistanceBiochemical Journal, 1969
- Some enzymic activities and chemical properties of the mesosomes and cytoplasmic membranes of Bacillus licheniformis 6346Biochemical Journal, 1969
- Membrane Attachment of the Chromosome Replication Origin in Bacillus subtilisCold Spring Harbor Symposia on Quantitative Biology, 1968
- Shear Sensitivity of the E. coli Genome: Multiple Membrane Attachment Points of the E. coli DNACold Spring Harbor Symposia on Quantitative Biology, 1968
- Properties of the growing point region in the bacterial chromosomeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- Studies on the phospholipids and morphology of protoplasts of Bacillus megateriumBiochimica et Biophysica Acta (BBA) - Biomembranes, 1967
- ISOLATION AND CHARACTERIZATION OF BACTERIAL MEMBRANES*,†Transactions of the New York Academy of Sciences, 1967
- Evidence for an intermediate stage in the biosynthesis of the Salmonella O-antigen.Proceedings of the National Academy of Sciences, 1965