BEHAVIOR OF MURINE LYMPHOCYTES AFTER INVITRO TREATMENT WITH ACID MUCOPROTEINS FROM HUMAN-SERUM

  • 1 January 1976
    • journal article
    • research article
    • Vol. 35  (6) , 813-817
Abstract
Seromucoproteins from human serum were isolated by perchloric acid extraction followed by DEAE-Sephadex A-50 ion exchange chromatography. The in vitro pretreatment of spleen leukocytes with this fraction caused a dose-dependent inhibition of graft-vs.-host [gvh] reaction and an increase of their electrophoretic mobility, the viability being maintained. Pretreatment of mice (prospective spleen cell donors or recipients of sheep red blood cells) with human seromucoproteins had no effect on the gvh reaction or on the agglutinin formation to sheep red blood cells under the given conditions. The suppressive effect after in vitro pretreatment may be attributed to a coating effect of seromucoproteins. The fact that spleen cells pretreated in vitro with seromucoproteins are lysed in the presence of complement and antiseromucoprotein antiserum supports this conclusion. These findings and data from the literature indicate that local concentrated mucoproteins in the skin graft bed in cases of protractedly surviving skin grafts, in the placenta and on neoplastic tissues can influence non-specifically the immune response. Understanding of this mechanism may open new possibilities in prolonging allograft survival time.

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