Summary The presence of folate conjugase(s) was established in cabbage. The conjugase(s) was found to have its pH optimum at 5.0 and 8.0 in acetate buffer and Tris buffer, respectively. At pH 8.0 this conjugase was able to split down to third gamma-linkage yielding pteroyltriglutamate while the polyglutamates were split to monoglutamate at pH 5.0.