The Relation Between the Growth of Mycobacterium tuberculosis and the Yield of Tuberculin on Synthetic Media

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Abstract
M. tuberculosis, H 37, was grown on Long''s synthetic and 3 ammonium malate media and the wt. of organisms, amt. of protein in the culture fluid, and pH detd. at 6 and 10 wks. A medium with ammonium malate, glucose and sucrose as N and C sources, with a pH of 7.8 at 6 wks., produced over twice as much tuberculin as media giving twice the bulk of organisms at a terminally acid pH. Long''s medium after 4 wks. growth was adjusted from pH 5.8 to 7.2 and incubated 2 wks. longer; twice as much tuberculo-protein was produced as in unadjusted flasks. The tuberculin prepared from alkaline media was found fully potent and specific by skin tests in tuberculous guinea pigs.

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